Laboratory of Chemical Synthesis
(Lodz University of Technology)
Prof. dr hab. Jerzy Gębicki and his team for many years have been involved in the synthesis and testing of chemical reactivity of a wide range of pyridinium salts. As confirmed by the results of research conducted by the team of prof. dr hab. Stefan Chłopicki, many of these compounds showed effects on the vascular endothelium. Pyridinium salt-oriented research was the starting point for JCET’s research and development works, carried out under the POIG 1.1.2 strategic research project. As a part of the project coordinated by JCET (POIG 2.2), the research infrastructure of the Chemical Synthesis Laboratory at the Lodz University of Technology was created.
Scale chemical synthesis and the adequate equipment for analytical and structural identification. The synthesis of labeled compounds, both with stable and radioactive isotopes, is also feasible. The laboratory also develops analytical procedures to identify quantitatively the presence of selected pyridine derivatives in plant extracts and other natural sources. Unique research techniques are developed to study the chemical principles of oxidative stress.
The reactions with reactive oxygen species can be studied kinetically by the pulse radiolytic technique. Novel probes for the detection of hydrogen peroxide, hypochlorous acid and peroxynitrite using colorimetric and fluorimetric methods are also developed.
Head of the Laboratory:
Professor Andrzej Marcinek, Ph.D.
Faculty of Chemistry, Lodz University of Technology
Major laboratory equipment:
Preparative HPLC Chromatograph (semipreparative liquid chromatograph Waters Purification System) with a quaternary pump, UV-Vis detector and fraction collector. The pressure reactor, glovebox for anaerobic experiments, specialist rotary evaporator,automatic vacuum dryer are also available.
Ultra Performance Liquid Chromatograph (UPLC) Aquity (Waters) coupled to LCT Premier XE mass spectrometer (Waters MicroMass) with TOF type analyzer, ESI or APCI ionization. The system may also work with fluorescence and photodiode (200-500 nm)detectors.
Agilent 7890A Gas chromatograph coupled to GCT Premier mass spectrometer (WatersMicroMass). The system is equipped with electron impact (EI), chemical ionization (CI), field ionization (FI) and field desorption (FD) ion sources.
HPLC Ultimate 3000 Liquid Chromatograph (Dionex) with electrochemical detector (ESA CoulArray).
Linear electron accelerator ELU-6e equipped with the novel optical detection system allows the identification and evaluation of reactive intermediates, especially radical products generated by oxidation or reduction in one-electron transfer processes.
Varian Cary 300 Spectrophotometer (Agilent 8453) and Varian Cary Eclipse Fluorescence Spectrophotometer along with a SX20 Stopped-Flow Spectrometer allow the studies of “slow” reactions and reactions monitored by low temperature matrix isolation.