{"id":1265,"date":"2015-12-04T15:25:00","date_gmt":"2015-12-04T14:25:00","guid":{"rendered":"http:\/\/jcet.eu\/new\/?p=1265"},"modified":"2016-05-18T12:15:37","modified_gmt":"2016-05-18T10:15:37","slug":"konik-morski-w-jcet","status":"publish","type":"post","link":"https:\/\/jcet.eu\/new\/2015\/12\/04\/konik-morski-w-jcet\/","title":{"rendered":"„Konik morski” w JCET"},"content":{"rendered":"

D\u0142ugo na niego czekali\u015bmy, ale pojawi\u0142 si\u0119! Analizator p\u0142yn\u00f3w zewn\u0105trzkom\u00f3rkowych Seahorse XFe96 ju\u017c dzia\u0142a!<\/strong> S\u0142u\u017cy do jednoczesnych pomiar\u00f3w w kom\u00f3rkach in vitro<\/em> oddychania mitochondrialnego (poprzez pomiar szybko\u015bci konsumpcji tlenu, OCR) oraz glikolizy (poprzez pomiar szybko\u015bci zewn\u0105trzkom\u00f3rkowego zakwaszenia, ECAR).<\/p>\n

Dzi\u0119ki temu pozwala na badanie:<\/p>\n

    \n
  1. fenotypu metabolicznego kom\u00f3rek,<\/li>\n
  2. funkcjonalno\u015bci ich mitochondri\u00f3w oraz aktywno\u015bci glikolitycznej, a tak\u017ce<\/li>\n
  3. zale\u017cno\u015bci kom\u00f3rek od r\u00f3\u017cnych substrat\u00f3w metabolicznych, takich jak glukoza, kwasy t\u0142uszczowe czy glutamina.<\/li>\n<\/ol>\n

    Seahorse XFe96<\/strong> umo\u017cliwia monitorowanie zmian jako\u015bciowych kom\u00f3rek w hodowlach pierwotnych lub ustabilizowanych pod wp\u0142ywem r\u00f3\u017cnego rodzaju interwencji.<\/strong> Opr\u00f3cz kom\u00f3rek przylegaj\u0105cych do pod\u0142o\u017ca badaniom mog\u0105 zosta\u0107 poddane tak\u017ce mitochondria (izolowane lub w kom\u00f3rkach z b\u0142on\u0105 kom\u00f3rkow\u0105 permeabilizowan\u0105 przy u\u017cyciu digitoniy), sferoidy, skrawki tkanek lub odpowiednio ma\u0142e organizmy. Wachlarz mo\u017cliwo\u015bci jest tym wi\u0119kszy, \u017ce analizator jest przystosowany do p\u0142ytek 96-do\u0142kowych<\/strong>, co pozwala na zaplanowanie wielu grup pomiarowych w ramach pojedynczego eksperymentu<\/strong>.<\/p>\n

    W celu zg\u0142\u0119bienia mo\u017cliwo\u015bci, jakie niesie ze sob\u0105 praca z analizatorem Seahorse XFe96, zach\u0119camy do zapoznania si\u0119 z wynikami pracy dr Patrycji Kaczary pt.: „Carbon monoxide released by CORM-401 uncouples mitochondrial respiration and inhibits glycolysis in endothelial cells: A role for mitoBKCa channels”<\/a><\/em>, kt\u00f3ra jest naszym ekspertem w dziedzinie\u00a0 badania aktywno\u015bci mitochondrialnej kom\u00f3rek \u015br\u00f3db\u0142onka.<\/p>\n

    \"S1\"<\/a><\/p>\n

     <\/p>\n

     <\/p>\n

     <\/p>\n

     <\/p>\n

     <\/p>\n

     <\/p>\n

     <\/p>\n

    \"S2\"<\/a><\/p>\n

     <\/p>\n

     <\/p>\n

     <\/p>\n

     <\/p>\n

     <\/p>\n

     <\/p>\n

     <\/p>\n

     <\/p>\n

    Producent: Seahorse Bioscience<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

    D\u0142ugo na niego czekali\u015bmy, ale pojawi\u0142 si\u0119! Analizator p\u0142yn\u00f3w zewn\u0105trzkom\u00f3rkowych Seahorse XFe96 ju\u017c dzia\u0142a! S\u0142u\u017cy do jednoczesnych pomiar\u00f3w w kom\u00f3rkach in vitro oddychania mitochondrialnego (poprzez pomiar szybko\u015bci konsumpcji tlenu, OCR) oraz glikolizy (poprzez pomiar szybko\u015bci zewn\u0105trzkom\u00f3rkowego zakwaszenia, ECAR). Dzi\u0119ki temu<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[11,10,8,9,6,7],"class_list":["post-1265","post","type-post","status-publish","format-standard","hentry","category-viaria","tag-glikoliza","tag-jcet","tag-komorka","tag-metabolizm","tag-mitochondrium","tag-seahorse"],"acf":[],"yoast_head":"\n"Konik morski" w JCET » JCET<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/jcet.eu\/new\/2015\/12\/04\/konik-morski-w-jcet\/\" \/>\n<meta property=\"og:locale\" content=\"pl_PL\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\""Konik morski" w JCET » JCET\" \/>\n<meta property=\"og:description\" content=\"D\u0142ugo na niego czekali\u015bmy, ale pojawi\u0142 si\u0119! Analizator p\u0142yn\u00f3w zewn\u0105trzkom\u00f3rkowych Seahorse XFe96 ju\u017c dzia\u0142a! S\u0142u\u017cy do jednoczesnych pomiar\u00f3w w kom\u00f3rkach in vitro oddychania mitochondrialnego (poprzez pomiar szybko\u015bci konsumpcji tlenu, OCR) oraz glikolizy (poprzez pomiar szybko\u015bci zewn\u0105trzkom\u00f3rkowego zakwaszenia, ECAR). Dzi\u0119ki temu\" \/>\n<meta property=\"og:url\" content=\"https:\/\/jcet.eu\/new\/2015\/12\/04\/konik-morski-w-jcet\/\" \/>\n<meta property=\"og:site_name\" content=\"JCET\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/jcet.eu\/\" \/>\n<meta property=\"article:published_time\" content=\"2015-12-04T14:25:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2016-05-18T10:15:37+00:00\" \/>\n<meta property=\"og:image\" content=\"http:\/\/jcet.eu\/new\/wp-content\/uploads\/2015\/12\/S1-300x168.jpg\" \/>\n<meta name=\"author\" content=\"Agnieszka Gudek\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Napisane przez\" \/>\n\t<meta name=\"twitter:data1\" content=\"Agnieszka Gudek\" \/>\n\t<meta name=\"twitter:label2\" content=\"Szacowany czas czytania\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minuta\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/jcet.eu\\\/new\\\/2015\\\/12\\\/04\\\/konik-morski-w-jcet\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/jcet.eu\\\/new\\\/2015\\\/12\\\/04\\\/konik-morski-w-jcet\\\/\"},\"author\":{\"name\":\"Agnieszka Gudek\",\"@id\":\"https:\\\/\\\/jcet.eu\\\/new\\\/#\\\/schema\\\/person\\\/13f485a795141f65ed056cb5e2b88f9e\"},\"headline\":\"„Konik morski” w JCET\",\"datePublished\":\"2015-12-04T14:25:00+00:00\",\"dateModified\":\"2016-05-18T10:15:37+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/jcet.eu\\\/new\\\/2015\\\/12\\\/04\\\/konik-morski-w-jcet\\\/\"},\"wordCount\":269,\"publisher\":{\"@id\":\"https:\\\/\\\/jcet.eu\\\/new\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/jcet.eu\\\/new\\\/2015\\\/12\\\/04\\\/konik-morski-w-jcet\\\/#primaryimage\"},\"thumbnailUrl\":\"http:\\\/\\\/jcet.eu\\\/new\\\/wp-content\\\/uploads\\\/2015\\\/12\\\/S1-300x168.jpg\",\"keywords\":[\"glikoliza\",\"jcet\",\"kom\u00f3rka\",\"metabolizm\",\"mitochondrium\",\"seahorse\"],\"articleSection\":[\"Varia\"],\"inLanguage\":\"pl-PL\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/jcet.eu\\\/new\\\/2015\\\/12\\\/04\\\/konik-morski-w-jcet\\\/\",\"url\":\"https:\\\/\\\/jcet.eu\\\/new\\\/2015\\\/12\\\/04\\\/konik-morski-w-jcet\\\/\",\"name\":\"\\\"Konik morski\\\" w JCET » JCET\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/jcet.eu\\\/new\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/jcet.eu\\\/new\\\/2015\\\/12\\\/04\\\/konik-morski-w-jcet\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/jcet.eu\\\/new\\\/2015\\\/12\\\/04\\\/konik-morski-w-jcet\\\/#primaryimage\"},\"thumbnailUrl\":\"http:\\\/\\\/jcet.eu\\\/new\\\/wp-content\\\/uploads\\\/2015\\\/12\\\/S1-300x168.jpg\",\"datePublished\":\"2015-12-04T14:25:00+00:00\",\"dateModified\":\"2016-05-18T10:15:37+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/jcet.eu\\\/new\\\/2015\\\/12\\\/04\\\/konik-morski-w-jcet\\\/#breadcrumb\"},\"inLanguage\":\"pl-PL\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/jcet.eu\\\/new\\\/2015\\\/12\\\/04\\\/konik-morski-w-jcet\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"pl-PL\",\"@id\":\"https:\\\/\\\/jcet.eu\\\/new\\\/2015\\\/12\\\/04\\\/konik-morski-w-jcet\\\/#primaryimage\",\"url\":\"https:\\\/\\\/jcet.eu\\\/new\\\/wp-content\\\/uploads\\\/2015\\\/12\\\/S1.jpg\",\"contentUrl\":\"https:\\\/\\\/jcet.eu\\\/new\\\/wp-content\\\/uploads\\\/2015\\\/12\\\/S1.jpg\",\"width\":1632,\"height\":916},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/jcet.eu\\\/new\\\/2015\\\/12\\\/04\\\/konik-morski-w-jcet\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Strona g\u0142\u00f3wna\",\"item\":\"https:\\\/\\\/jcet.eu\\\/new\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"„Konik morski” w JCET\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/jcet.eu\\\/new\\\/#website\",\"url\":\"https:\\\/\\\/jcet.eu\\\/new\\\/\",\"name\":\"JCET\",\"description\":\"Centrum doskona\u0142o\u015bci w farmakologii \u015br\u00f3db\u0142onka.\",\"publisher\":{\"@id\":\"https:\\\/\\\/jcet.eu\\\/new\\\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/jcet.eu\\\/new\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"pl-PL\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/jcet.eu\\\/new\\\/#organization\",\"name\":\"Jagiello\u0144skie Centrum Rozwoju Lek\u00f3w (JCET)\",\"url\":\"https:\\\/\\\/jcet.eu\\\/new\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"pl-PL\",\"@id\":\"https:\\\/\\\/jcet.eu\\\/new\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"http:\\\/\\\/jcet.eu\\\/new\\\/wp-content\\\/uploads\\\/2022\\\/06\\\/JCET-logo.png\",\"contentUrl\":\"http:\\\/\\\/jcet.eu\\\/new\\\/wp-content\\\/uploads\\\/2022\\\/06\\\/JCET-logo.png\",\"width\":715,\"height\":401,\"caption\":\"Jagiello\u0144skie Centrum Rozwoju Lek\u00f3w (JCET)\"},\"image\":{\"@id\":\"https:\\\/\\\/jcet.eu\\\/new\\\/#\\\/schema\\\/logo\\\/image\\\/\"},\"sameAs\":[\"https:\\\/\\\/www.facebook.com\\\/jcet.eu\\\/\"]},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/jcet.eu\\\/new\\\/#\\\/schema\\\/person\\\/13f485a795141f65ed056cb5e2b88f9e\",\"name\":\"Agnieszka Gudek\",\"url\":\"https:\\\/\\\/jcet.eu\\\/new\\\/author\\\/agag\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"\"Konik morski\" w JCET » JCET","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/jcet.eu\/new\/2015\/12\/04\/konik-morski-w-jcet\/","og_locale":"pl_PL","og_type":"article","og_title":"\"Konik morski\" w JCET » JCET","og_description":"D\u0142ugo na niego czekali\u015bmy, ale pojawi\u0142 si\u0119! Analizator p\u0142yn\u00f3w zewn\u0105trzkom\u00f3rkowych Seahorse XFe96 ju\u017c dzia\u0142a! S\u0142u\u017cy do jednoczesnych pomiar\u00f3w w kom\u00f3rkach in vitro oddychania mitochondrialnego (poprzez pomiar szybko\u015bci konsumpcji tlenu, OCR) oraz glikolizy (poprzez pomiar szybko\u015bci zewn\u0105trzkom\u00f3rkowego zakwaszenia, ECAR). Dzi\u0119ki temu","og_url":"https:\/\/jcet.eu\/new\/2015\/12\/04\/konik-morski-w-jcet\/","og_site_name":"JCET","article_publisher":"https:\/\/www.facebook.com\/jcet.eu\/","article_published_time":"2015-12-04T14:25:00+00:00","article_modified_time":"2016-05-18T10:15:37+00:00","og_image":[{"url":"http:\/\/jcet.eu\/new\/wp-content\/uploads\/2015\/12\/S1-300x168.jpg","type":"","width":"","height":""}],"author":"Agnieszka Gudek","twitter_card":"summary_large_image","twitter_misc":{"Napisane przez":"Agnieszka Gudek","Szacowany czas czytania":"1 minuta"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/jcet.eu\/new\/2015\/12\/04\/konik-morski-w-jcet\/#article","isPartOf":{"@id":"https:\/\/jcet.eu\/new\/2015\/12\/04\/konik-morski-w-jcet\/"},"author":{"name":"Agnieszka Gudek","@id":"https:\/\/jcet.eu\/new\/#\/schema\/person\/13f485a795141f65ed056cb5e2b88f9e"},"headline":"„Konik morski” w JCET","datePublished":"2015-12-04T14:25:00+00:00","dateModified":"2016-05-18T10:15:37+00:00","mainEntityOfPage":{"@id":"https:\/\/jcet.eu\/new\/2015\/12\/04\/konik-morski-w-jcet\/"},"wordCount":269,"publisher":{"@id":"https:\/\/jcet.eu\/new\/#organization"},"image":{"@id":"https:\/\/jcet.eu\/new\/2015\/12\/04\/konik-morski-w-jcet\/#primaryimage"},"thumbnailUrl":"http:\/\/jcet.eu\/new\/wp-content\/uploads\/2015\/12\/S1-300x168.jpg","keywords":["glikoliza","jcet","kom\u00f3rka","metabolizm","mitochondrium","seahorse"],"articleSection":["Varia"],"inLanguage":"pl-PL"},{"@type":"WebPage","@id":"https:\/\/jcet.eu\/new\/2015\/12\/04\/konik-morski-w-jcet\/","url":"https:\/\/jcet.eu\/new\/2015\/12\/04\/konik-morski-w-jcet\/","name":"\"Konik morski\" w JCET » JCET","isPartOf":{"@id":"https:\/\/jcet.eu\/new\/#website"},"primaryImageOfPage":{"@id":"https:\/\/jcet.eu\/new\/2015\/12\/04\/konik-morski-w-jcet\/#primaryimage"},"image":{"@id":"https:\/\/jcet.eu\/new\/2015\/12\/04\/konik-morski-w-jcet\/#primaryimage"},"thumbnailUrl":"http:\/\/jcet.eu\/new\/wp-content\/uploads\/2015\/12\/S1-300x168.jpg","datePublished":"2015-12-04T14:25:00+00:00","dateModified":"2016-05-18T10:15:37+00:00","breadcrumb":{"@id":"https:\/\/jcet.eu\/new\/2015\/12\/04\/konik-morski-w-jcet\/#breadcrumb"},"inLanguage":"pl-PL","potentialAction":[{"@type":"ReadAction","target":["https:\/\/jcet.eu\/new\/2015\/12\/04\/konik-morski-w-jcet\/"]}]},{"@type":"ImageObject","inLanguage":"pl-PL","@id":"https:\/\/jcet.eu\/new\/2015\/12\/04\/konik-morski-w-jcet\/#primaryimage","url":"https:\/\/jcet.eu\/new\/wp-content\/uploads\/2015\/12\/S1.jpg","contentUrl":"https:\/\/jcet.eu\/new\/wp-content\/uploads\/2015\/12\/S1.jpg","width":1632,"height":916},{"@type":"BreadcrumbList","@id":"https:\/\/jcet.eu\/new\/2015\/12\/04\/konik-morski-w-jcet\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Strona g\u0142\u00f3wna","item":"https:\/\/jcet.eu\/new\/"},{"@type":"ListItem","position":2,"name":"„Konik morski” w JCET"}]},{"@type":"WebSite","@id":"https:\/\/jcet.eu\/new\/#website","url":"https:\/\/jcet.eu\/new\/","name":"JCET","description":"Centrum doskona\u0142o\u015bci w farmakologii \u015br\u00f3db\u0142onka.","publisher":{"@id":"https:\/\/jcet.eu\/new\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/jcet.eu\/new\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"pl-PL"},{"@type":"Organization","@id":"https:\/\/jcet.eu\/new\/#organization","name":"Jagiello\u0144skie Centrum Rozwoju Lek\u00f3w (JCET)","url":"https:\/\/jcet.eu\/new\/","logo":{"@type":"ImageObject","inLanguage":"pl-PL","@id":"https:\/\/jcet.eu\/new\/#\/schema\/logo\/image\/","url":"http:\/\/jcet.eu\/new\/wp-content\/uploads\/2022\/06\/JCET-logo.png","contentUrl":"http:\/\/jcet.eu\/new\/wp-content\/uploads\/2022\/06\/JCET-logo.png","width":715,"height":401,"caption":"Jagiello\u0144skie Centrum Rozwoju Lek\u00f3w (JCET)"},"image":{"@id":"https:\/\/jcet.eu\/new\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.facebook.com\/jcet.eu\/"]},{"@type":"Person","@id":"https:\/\/jcet.eu\/new\/#\/schema\/person\/13f485a795141f65ed056cb5e2b88f9e","name":"Agnieszka Gudek","url":"https:\/\/jcet.eu\/new\/author\/agag\/"}]}},"_links":{"self":[{"href":"https:\/\/jcet.eu\/new\/wp-json\/wp\/v2\/posts\/1265","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/jcet.eu\/new\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/jcet.eu\/new\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/jcet.eu\/new\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/jcet.eu\/new\/wp-json\/wp\/v2\/comments?post=1265"}],"version-history":[{"count":13,"href":"https:\/\/jcet.eu\/new\/wp-json\/wp\/v2\/posts\/1265\/revisions"}],"predecessor-version":[{"id":1944,"href":"https:\/\/jcet.eu\/new\/wp-json\/wp\/v2\/posts\/1265\/revisions\/1944"}],"wp:attachment":[{"href":"https:\/\/jcet.eu\/new\/wp-json\/wp\/v2\/media?parent=1265"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/jcet.eu\/new\/wp-json\/wp\/v2\/categories?post=1265"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/jcet.eu\/new\/wp-json\/wp\/v2\/tags?post=1265"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}