PROJECT MANAGER

Aneta Radziwon-Balicka, PhD, DSc
Assistant Professor/Adjunct
aneta.radziwon-balicka@uj.edu.pl

Field: thrombosis, haemostasis and vascular biology, translational research, platelet research.
ResearchGate Profile

I finished my PhD studies in 2010 at the Department of Pharmacology in Trinity College Dublin, Ireland. My PhD studies focused on platelet-cancer cell interactions, since patients with cancer often have activated haemostatic systems. To further my training in the research, I have joined the Department of Pharmacy and Pharmaceutical Sciences at the University of Alberta, Canada for my postdoctoral training. During this time, I have discovered two platelet subpopulations based on the presence or absence of eNOS and a functional NO-cGMP signaling pathway. This translational study established the basic biology and clinical significance of platelet subpopulations based on the presence of a functional eNOS-signaling pathway by better predicting risk and extent of thrombi formation. Next, I have started postdoctoral training at Glostrup Research Institute in Denmark, where I was studying pathophysiology of vasculature after stroke incident. In 2020 I have recived habilitation from Medical University of Wroclaw, Poland.  In addition, my research at the North Zealand Hospital in Denmark was focused on the development of a novel blood anticoagulant together with the private company collaboration. I am a grant recipient of PolonezBis from the National Sciente Center Poland, which is going to investigate the role of platelets on endothelial barrier integrity: “Effects of tyrosine kinase inhibitors on Syk pathway in eNOS-based platelet subpopulations and on endothelial barrier integrity”.

PROJECT SUMMARY:

The small molecule inhibitors represent an era of target-directed therapy and precision oncology used in the treatment of various malignancies. Despite the success of this therapy, they can induce side effects such as thrombosis, bleeding, and cardiovascular events. A better understanding of the pathogenesis of cardiovascular complications in patients treated with small molecule inhibitors is urgently needed to improve safety of cancer therapy. In this project we aim to characterize a possible effect of platelets on endothelial cells, to study effects of small molecule inhibitors on functionality of endothelium, and finally, to perform proof of principle, clinical study investigating whether platelets from chronic myeloid leukemia patients untreated or treated with small molecule inhibitors may have different effect on platelets compared to the healthy individuals. We anticipate that this research proposal will bring a novel knowledge regarding platelets and their functional effect on endothelial barrier integrity. In addition, the clinical study will test whether chronic myeloid leukemia patients untreated and treated with small molecule inhibitors may have different effect on platelets and on endothelial barrier integrity.

 

MAJOR  METHODS

  • Flow cytometry (intra-, extra-cellular)
  • platelet aggregometry
  • QCM-D (quartz crystal microbalance with dissipation),
  • confocal microscopy
  • Incucyte
  • zymography
  • MSD

PUBLICATIONS

Selected publications:

  1. Radziwon-Balicka A, Lesyk G, Back V, Fong T, Loredo-Calderon EL, Dong B, El-Sikhry H, El-Sherbeni AA, El-Kadi A, Ogg S, Siraki A, Seubert JM, Santos-Martinez MJ, Radomski MW, Velazquez-Martinez CA, Winship IR, Jurasz P. Differential eNOS-signalling by platelet subpopulations regulates adhesion and aggregation. Cardiovasc Res. 2017 Dec 1;113(14):1719-1731. doi: 10.1093/cvr/cvx179. PMID: 29016749; PMCID: PMC5852541.

  2. Radziwon-Balicka A, Santos-Martinez MJ, Corbalan JJ, O’Sullivan S, Treumann A, Gilmer JF, Radomski MW, Medina C. Mechanisms of platelet-stimulated colon cancer invasion: role of clusterin and thrombospondin 1 in regulation of the P38MAPK-MMP-9 pathway. Carcinogenesis. 2014 Feb;35(2):324-32. doi: 10.1093/carcin/bgt332. Epub 2013 Oct 1. PMID: 24085798.

ORCID: 0000-0002-5123-57 10

NEWS

2024

Untitled designJuny – 13-14.06.2024  two events took place at JCET: an Open Day and a Meeting with Students from the UJ CM Doctoral School. Both events included a lecture by Aneta Radziwon-Balicka, PhD, who explained what the work of a researcher looks like in the example of the Polonez Bis project funded by the National Science Centre. 

Untitled designMay – The official welcoming of a new member of the JCET Team, Aneta Radziwon-Balicka, Ph.D., who will be carrying out a project entitled: “Effects of tyrosine kinase inhibitors on Syk pathway in eNOS-based platelet subpopulations and on endothelial barrier integrity“, under funding from the POLONEZ BIS-3 programme.