JCET

Modern biology is very diverse with a plethora of sophisticated techniques that utilize reagents that are specifically designed to support those techniques and come in the form specialized media, labeled reagents, tool compounds, antibodies, fluorescent probes, PCR primers, a multitude of kits and disposable items such as columns to name but a few. To increase the quality of our doctoral students research projects, they know what they want to do to get better data but are frequently limited by the cost of these essential reagents that result in either not doing the experiment or choosing a cheaper option to get data that is okay but will not reach the threshold to get better publications.
Therefore for the Biomedical Doctoral School that resides within the Natural and Exact Sciences, is requesting to use the ID.UJ Research Support Module money for reagent and disposable item purchases for our doctoral students. The awards of 13000 PLN will go to up to 10 students that give the strongest rationale for how the reagent money and disposable items can improve their quality of research. The remaining small portion of money will be for administrative support to process invoices.

• Details of regulations in the attachment
• Form for research module 2022

PhD Kamil Przyborowski is the third laureate of the last edition of the SONATA competition, announced by the National Center of Science in Krakow on behalf of the Jagiellonian Centre for Experimental Therapeutics (JCET). Doctor Przyborowski will implement a project entitled Inhibition of protein disulfide isomerases as a strategy for reduction of prothrombotic state in atherosclerosis; therapeutic implications.

Antiplatelet treatment is a gold standard to combat atherothrombosis. Despite advances in antithrombotic therapy the cardiovascular diseases (CVDs), including myocardial infarction and stroke, are still the leading causes of death worldwide. Therefore, development of more effective and safe antithrombotic agents targeting new mechanisms is crucial. Protein disulfide isomerase (PDI) A1, PDIA3 or PDIA6 released from platelets and endothelium accelerate thrombus formation through effects on platelets and coagulation. Consequently, they are emerging as targets to inhibit thrombosis.

In the 17th edition of the SONATA 17 competition announced by the National Science Center in Krakow, PhD Agnieszka Kij is the second scientist from the Jagiellonian Centre for Experimental Therapeutics (JCET) which to receive funding for research . The Doctor Kij will run a project entitled “In the search of specific oxylipin fingerprint reflecting the development of endothelial dysfunction with the use of non-targeted and targeted lipidomics”.

Oxylipins are biologically active lipid mediators encompassing oxygenation products of polyunsaturated fatty acids (PUFAs) including arachidonic acid (AA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Oxylipins such as AA-derived eicosanoids participate mainly in pro-inflammatory response (e.g., prostaglandins), with the exception of vasoprotective prostacyclin (PGI2). In recent years, an increasing attention has been paid to a novel class of AA-, EPA- and DHA-derived oxylipins referred as specialized pro-resolving mediators (SPMs; e.g., lipoxins LX, maresins MaR, resolvins RvE and RvD), that govern the resolution of inflammation and promote tissue regeneration. One can assume, that unresolved inflammation resulted from failed lipid mediator class switching from pro-inflammatory eicosanoids to SPMs can contribute to chronic vascular inflammation, and consequently to coronary and systemic endothelial dysfunction (ED). Despite the growing knowledge on lipid-dependent molecular mechanisms involved in endothelial dysfunction development, the pattern and time-frame of oxylipin alterations linked specifically to hyperlipidaemia- and age-induced coronary and systemic endothelial dysfunction, that could be helpful for early diagnosis, prevention and treatment of ED and cardiovascular diseases (e.g., atherosclerosis), have not been characterized.