Laboratory of EPR Spectroscopy (JCET)
The laboratory of EPR spectroscopy conducts research focused on oxidative and nitrosative stress in models of endothelium dysfunction in vitro, ex vivo and in vivo. EPR methods allow sensitive and highly specific detection of reactive oxygen species (ROS), such as superoxide, as well nitric oxide that are produced by the endothelium and vascular wall. They are successfully used for researching various pathological states as well as testing therapeutical strategies in cell cultures and animal models. For the research, the laboratory also utilizes complementary and tests novel methods for ROS and NO detection based on chemiluminescence, fluorescent probes, HPLC and immuno-spin trapping.
EMX+ EPR Spectrometer (Bruker) allows cw-EPR measurements in liquid nitrogen (77 K) using finger dewar, as well as at temperatures in the range of 100-500K using a temperature controller.
E-scan spectrometer (Bruker) designed for standard measurements of free radical signals (g » 2). It can be used for clinical application (measuring free radicals, including NO) and for industrial tests (evaluation of food, cosmetics shelf life, etc.)
Spectrophotometer Lambda 950 (Perkin Elmer) – double-beam with double monochromator and regulated reference beam energy corresponding to wavelengths in the range of 185-3300 nm. It allows scanning and measuring kinetics simultaneously for 8 different wavelengths. It is equipped with the cuvette temperature control system PTP6 that allows setting the reaction temperature in the range 0-110°C.
Sievers nitric oxide analyzer designed for detection of nitric oxide in the exhaled air and measurements of nitrite and nitrate concentrations in body fluids and other biological samples (blood, cell cultures, tissues, etc.). Sensitivity limit for gas samples reaches 0,5 ppb, in liquid samples reaches the concentration as low as 1 pM.
Luminometer LB9508 (Berthold Technologies) with a detector for a single photon counting (photomultiplier), designed for standard chemiluminescence and bioluminescence measurements in flash and glow mode and for LIA analyses in the sampling mode with the sensitivity of the order of 1 attomol ATP.