Katarzyna M. Marzec, PhD, DSc
Assistant Professor/Adjunct
phone: (+48 12) 6635476
Field: chemistry
ResearchGate Profile



Katarzyna Bulat, PhD, BEng
phone: (+48 12) 297 5472
room: B113
Field: chemical engineering
ResearchGate Profile



Magdalena Kaczmarska, PhD
phone: (+48 12) 297 5472
room: B113
Field: physics
ResearchGate Profile



Ewa Szczęsny-Małysiak, PhD
phone: (+48 12) 297 5472
room: B113
Field: medical biology
ResearchGate Profile



Jakub Dybas, PhD
phone: (+48 12) 297 5472
room: B113
Field: chemistry
ResearchGate Profile



Aleksandra Wajda, PhD, BEng
phone: (+48 12) 297 5472
room: B113
Field: material engineering
ResearchGate Profile


Fatih Celal Alçiçek, MD
PhD student
phone: (+48 12) 297 5472
room: B114
Field: medicine
supervisor: dr hab. Katarzyna Marzec



Aneta Blat, MSc, PhD student
phone: +48 12 686 2391
Faculty of Chemistry, UJ, room: C3-23
PhD at Faculty of Chemistry, UJ
supervisor: dr hab. Kamilla Malek
Field: chemistry
ResearchGate Profile



Anna Zimna
Faculty of Pharmacy, CM UJ
supervisor: dr Magdalena Kaczmarska
Field: medical analytics



Tetiana Stepanenko
Faculty of Chemistry, UJ
Field: chemistry
ResearchGate Profile





Mateusz Mardyla, MSc
phone: (+48 12) 297 5472
room: B113
ResearchGate Profile


We are a part of Raman Research Group at JCET and we cooperate closely with Raman Imaging Group at the Faculty of Chemistry UJ headed by Prof. dr hab. Małgorzata Baranska.

We cooperate closely with whole JCET team headed by Prof. dr hab. Stefan Chlopicki.
Until now, we have been cooperating mainly with dr Marek Grosicki, dr Kamil Kuś and Tasnim Mohaissen.


1. Diagnostics of the biochemical alterations in packed human red blood cells (PRBCs) with the use of vibrational spectroscopy

Funding Agency: National Centre for Research and Development “Lider VIII”
Supervisor: dr hab. Katarzyna M. Marzec
Participants: dr Jakub Dybas; dr Magdalena Kaczmarska; dr Ewa Szczęsny
Budget: 1 200 000 PLN

2. In search of biochemical, mechanical and functional fingerprints of oxidative stress in RBCs

Funding Agency: National Science Centre, Poland, Program: OPUS
Supervisor: dr hab. Katarzyna M. Marzec
Participants: dr Katarzyna Bułat, dr Aleksandra Wajda, Aneta Blat, Tasnim Mohaissen
Budget: 1 239 900 PLN

3. Method for the measurement of the deformation-induced ATP release from RBCs based on prototype of the deformation-induced ATP releaser (DIAR)

Funding Agency: Centre for Technology Transfer CITTRU
PI: Fatih Celal Alçiçek; Supervisor: dr hab. Katarzyna M. Marzec
Budget: 50 000 PLN

4. Opracowanie metody indukcji sulfhemoglobinemii w funkcjonalnych, ludzkich erytrocytach i analiza struktury molekularnej układu porfirynowego wewnątrz„zielonych erytrocytów” (ang. Method of induction of sulfhemoglobinemia in functional human erythrocytes and analysis of the molecular structure of the porphyrin inside “green erythrocytes”.

Funding Agency: The BioS Priority Research Area – Strategic Program Excellence Initiative at the Jagiellonian University
PI: Tatiana Stepanenko; Supervisor: dr Jakub Dybas
Budget: 9 850 PLN


  • Application of the spectroscopy supported by complementary techniques to human and murine RBCs studies
  • RBCs stress response on the biochemical, mechanical and functional level
  • Functional and dysfunctional hemoglobin adducts structure and analysis
  • RBCs membrane and cytoskeleton alterations
  • RBCs function and alterations in lifestyle diseases and in cancer metastasis
  • RBCs aging


  • Vibrational Spectroscopy:
    –  Raman and Resonance Raman spectroscopy (RS, RRS)
    –  Fourier-transform infrared spectroscopy (FT-IR)
  • Atomic force microscopy (AFM)
  • Scanning near-field optical microscope (SNOM)
  • UV–Vis absorption spectroscopy
  • Ektacytometry
  • Flow cytometry
  • Other classical techniques of blood and RBCs analysis (Blood gas analysis, Complete Blood counter, Morphology, Measurements of biochemical parameters etc.)


  • 04.12.2020 – an international patent application PCT was filed at the Patent Office of the Republic of Poland,entitled Non–invasive and label–free method for evaluation of biochemical state of packed red blood cells, application number: PCT/PL2020/050093, J. Dybas, A. Wajda, K. Bułat, M. Kaczmarska, M. Grosicki, K.M. Marzec.
  • 24.11.2020 – a patent application was filed at the Patent Office of the Republic of Poland, entitled Sposób określania stanu biochemicznego koncentratów krwinek czerwonych z zastosowaniem spektroskopii absorpcyjnej w podczerwieni (eng. Method for determining the biochemical status of red blood cell concentrates with the use of infrared absorption spectroscopy), application number: P.436054, A. Blat, A. Wajda, E. Szczesny-Malysiak, K. Malek, K.M.Marzec.
  • 12.12.2019 – a patent application was filed at the Patent Office of the Republic of Poland, entitled Sposób określania stanu biochemicznego koncentratu krwinek czerwonych (eng. Method for determining the biochemical state of the red blood concentrate), application number: P.43217, A. Wajda, J. Dybas, K. Bulat, M. Kaczmarska, M. Grosicki, K.M.Marzec.
  • 30.11.2018 – Trade mark registration (Patent Office of the Republic of Poland, no. Z.493251).


Selected publications related to RBC research:

  1. J. Dybas, T. Chiura, K.M. Marzec, P.J. Mak, Characterization of cyanide ligand binding to hemoglobin inside functional RBCs versus isolated protein by resonance Raman spectroscopy, The Journal of Physical Chemistry B 2021. Accepted, IF= 2,857, MNiSW=140.
  2. A. Zimna, M. Kaczmarska*, E. Szczesny-Malysiak, A. Wajda, K. Bulat, F. C. Alcicek, M. Zygmunt, T. Sacha, K.M. Marzec*, An insight into the stages of ion leakage during red blood cell storage, Int. J. Mol. Sci. 2021, 22(6), 2885, DOI: 10.3390/ijms22062885, IF= 4,556, MNiSW=140.
  3. A. Blat, T. Stepanenko, K. Bulat, A. Wajda, J. Dybas, T. Mohaissen, F.C. Alcicek, E. Szczesny-Malysiak, K. Malek, A. Fedorowicz, K.M. Marzec*, Spectroscopic Signature of Red Blood Cells in the D-galactose-Induced Accelerated Aging Model, Int. J. Mol. Sci. 2021, 22(5), 2660, DOI: 10.3390/ijms22052660, IF= 4,556, MNiSW=140.
  4. J. Dybas, K. Bulat, A. Blat, T. Mohaissen, A. Wajda, M. Mardyla, M. Kaczmarska, M. Franczyk-Zarow, K. Malek, S. Chlopicki, K.M. Marzec*, Age-related and atherosclerosis-related erythropathy in ApoE/LDLR‒/‒ miceBBA – Molecular Basis of Disease 2020, 1866 (12),165972,DOI: 10.1016/j.bbadis.2020.165972, IF=4,352, MNiSW=140.
  5. E. Szczęsny-Małysiak, J. Dybas, A. Blat, K. Bulat, K. Kuś, M. Kaczmarska, A. Wajda, K. Malek, S. Chlopicki, K. M. Marzec*,  Irreversible alterations in the hemoglobin structure affect oxygen binding in human packed red blood cells, BBA – Molecular Cell Research 2020,1867 (11),118803, DOI: 10.1016/j.bbamcr.2020.118803, IF=4,105, MNiSW=140.
  6. J. Dybas, M. J. Bokamper, K. M. Marzec*, P. J. Mak*, Probing the structure-function relationship of hemoglobin in living human red blood cells, Spectrochim. Acta A2020, 239, 118530,DOI: 10.1016/j.saa.2020.118530, IF=3,232, MNiSW=100.
  7. M. Kaczmarska, M. Grosicki, K. Bulat, M. Mardyla, E. Szczesny-Malysiak, A. Blat, J. Dybas, T. Sacha, K. M. Marzec*, Temporal sequence of the human RBCs’ vesiculation observed in nano-scale with application of AFM and complementary techniques, Nanomedicine: NBM 2020, 28,102221, DOI:10.1016/j.nano.2020.102221, IF=5,182, MNiSW=140.
  8. K. Bulat, J. Dybas, M. Kaczmarska, A. Rygula, A.Jasztal, E. Szczesny-Malysiak, M. Baranska, B. R. Wood, K. M. Marzec*,Multimodal detection and analysis of a new type of advanced Heinz body-like aggregate (AHBA)and cytoskeleton deformation in human RBCs, Analyst 2020,145,1749-1758, DOI:10.1039/C9AN01707K, IF=3,978, MNiSW=100.
  9. A. Blat, J. Dybas, K. Chrabaszcz, K. Bulat, A. Jasztal, M. Kaczmarska, T. Popiela, A. Slowik, K. Malek, M.G. Adamski, K.M. Marzec*, FTIR, Raman and AFM characterization of the clinically valid biochemical parameters of the thrombi in acute ischemic stroke, Sci.Rep. 2019, 9 (15475), DOI: 10.1038/s41598-019-51932-0, IF=4,011, MNiSW=140.
  10. A. Blat, J. Dybas, M. Kaczmarska, K. Chrabaszcz, K. Bulat, R.B. Kostogrys, A.Cernescu, K. Malek, K.M. Marzec*, An Analysis of Isolated and Intact RBC Membranes – a Comparison of a Semiquantitative Approach by Means of FTIR, Nano-FTIR and Raman Spectroscopies, Anal.Chem. 2019, 91(15), 9867-9874, DOI: 10.1021/acs.analchem.9b01536, IF=6,785, MNiSW=140.
  11. D. Perez–Guaita, K.M. Marzec, A. Hudson, C. Evans, T. Chernenko, C. Matthäus, M. Miljkovic, M. Diem, P. Heraud, J. Richards, D. Andrew, D. Anderson, C. Doerig, J. Garcia–Bustos, D. McNaughton, B.R. Wood*, Parasites under the spotlight: Applications of vibrational spectroscopy to malaria research, Chem. Rev. 2018, 118, 5330–5358, DOI: 10.1021/acs.chemrev.7b00661, IF=54,301, MNiSW=200.
  12. J. Dybas, P. Berkowicz, B. Proniewski, K. Dziedzic–Kocurek, J. Stanek, M. Barańska, S. Chłopicki*, K.M. Marzec*,Spectroscopy–based characterization of Hb–NO adducts in human red blood cells exposed to NO–donor and endothelium–derived NO, Analyst 2018, 143, 4335–4346, DOI: 10.1039/C8AN00302E, IF=3,976.
  13. J. Dybas, M. Grosicki, M. Barańska*, K.M. Marzec*,Raman imaging of haem metabolism in situ in macrophages and Kupffer cells, Analyst 2018,143, 3489–3498, DOI: 10.1039/C8AN00282G, IF=3,976.
  14. D. Perez–Guaita, M. de Veij, K.M. Marzec, A.R.D. Almohammedi, D. McNaughton, A.J. Hudson*, B.R. Wood*, Resonance Raman and UV–Visible Microscopy Reveals that Conditioning Red Blood Cells with Repeated Doses of Sodium Dithionite Increases Haemoglobin Oxygen Uptake, Chemistry Select 2017, 2(11), 3342–3346, DOI: 10.1002/slct.201700190, IF=1,520.
  15. M. Kaczmarska, D. Zydek, J. Wiklacz-Potoczny, M. Fornal, T. Gordzicki, E. Kochowska, K. Kozak, L. Gocal, W. Pohorecki, K. Matlak, J. Korecki, K. Burda, Influence of very small doses of alpha radiation on the stability of erythrocytes, Microscopy.Res.Tech. 2017, 80(1), 131-143, DOI: 10.1002/jemt.22803, IF=1,087.
  16. K.M. Marzec*, J. Dybas, S. Chłopicki, M. Barańska,Resonance Raman in vitro detection and differentiation of the nitrite–induced hemoglobin adducts in functional human red blood cells, J. Phys. Chem. B2016, 120, 12249–12260, DOI: 10.1021/acs.jpcb.6b08359, IF=3,177.
  17. S. Talu, S. Stach, M. Kaczmarska, M. Fornal, T. Grodzicki, W. Pohorecki, K. Burda*, Multifractal characterization of morphology of human red blood cells membrane skeleton,J.Microsc. 2016, 262(1), 59-72, DOI: 10.1111/jmi.12342, IF=1,692.
  18. M. Kaczmarska, I. Habina, A. Orzechowska, K. Niemiec-Murzyn, M. Fornal, W. Pohorecki, K. Matlak, J. Korecki, T. Grodzicki, K. Burda*, Influence of neutron radiation on the stability of the erythrocyte membrane and oxyhemoglobin formation – Petkau effect studies, Acta Phys.Pol.B 2016, 47(2), 425-440, DOI: 10.5506/APhysPolB.47.425, IF=0,904.
  19. K.M. Marzec*, A. Rygula, B.R. Wood, S. Chłopicki, M. Barańska, High–resolution Raman imaging reveals spatial location of heme oxidation sites in single RBCs of dried smears,J. Raman Spectrosc. 2015, 46, 76–83, DOI: 10.1002/jrs.4600, IF=2,395.
  20. K.M. Marzec,D. Perez–Guaita, M. de Veij, D. McNaughton, M. Barańska, M.W.A. Dixon, L. Tilley, B.R. Wood*, Red blood cells polarize green laser light revealing hemoglobin’s enhanced non–fundamental Raman modes, Chem.Phys.Chem. 2014, 15, 3963–3968, DOI: 10.1002/cphc.201402598, IF=3,419.  
  21. M. Kaczmarska, M. Fornal, F.H. Messerli, J. Korecki, T. Grodzicki, K. Burda*, Erythrocyte membrane properties in patients with essential hypertension, Cell Biochem.Biophys. 2013, 67(3), 1089-102, DOI: 10.1007/s12013-013-9613-9, IF=2,380.
  22. K. Niemiec, M. Kaczmarska, M. Buczkowski, M. Fornal, W. Pohorecki, K. Matlak, J. Korecki, T. Gordzicki, K. Burda*, Mössbauer studies of hemoglobin in erythrocytes exposed to neutron radiation, Hyperfine Interact. 2012, 206(1-3), 95-100, DOI: 10.1007/s10751-011-0491-5, IF=0,880.
  23. M. Kaczmarska, Z. Kopyscinska, M. Fornal, T. Gordzicki, K. Matlak, J. Korecki, K. Burda*, Effects of low doses of gamma rays on the stability of normal and diabetic erythrocytes, Acta Biochim.Pol. 2011, 58(4), 489-96, DOI: 10.18388/abp.2011_2215, IF=1,491.

Selected book chapters related to RBC research:

  1. B.R. Wood, K. Kochan, K.M. Marzec, Resonance Raman spectroscopy of hemoglobin in red blood cells, Chapter 13, Vibrational Spectroscopy in Protein Research Yukihiro Ozaki, Malgorzata Baranska, Igor Lednev, Bayden Wood (Eds.), Elsevier2020, pp 375-414, Paperback ISBN: 9780128186107, eBook ISBN: 9780128186114
  2. M. Marzec*, J. Dybaś, Resonance Raman scattering spectroscopyin Vibrational spectroscopy. From theory to practice. red. K. Małek, Wydawnictwo Naukowe PWN SA, 2016, Warszawa, str. 46–52 (ISBN: 978–83–01–18885–6).
    Wersja w jęz. Polskim:
    K.M. Marzec*, J. Dybaś, Spektroskopia rezonansowego rozpraszania ramanowskiego w Spektroskopia oscylacyjna. Od teorii do praktyki. red. K. Małek, Wydawnictwo Naukowe PWN SA, 2016, Warszawa, str. 47–53 (ISBN: 978–83–01–18826–9).
  3. Dybaś, A. Chmura–Skirlińska, K.M. Marzec*, Resonance Raman scattering spectroscopy inhemoglobin structure studiesin Vibrational spectroscopy. From theory to practice. red. K. Małek, Wydawnictwo Naukowe PWN SA, 2016, Warszawa, str. 185–192 (ISBN: 978–83–01–18885–6).
    Wersja w jęz. polskim:
    J. Dybaś, A. Chmura–Skirlińska, K.M. Marzec*, Spektroskopia rezonansowego rozpraszania ramanowskiego w badaniu struktury hemoglobiny w Spektroskopia oscylacyjna. Od teorii do praktyki. red. K. Małek, Wydawnictwo Naukowe PWN SA 2016, str. 191–198 (ISBN: 978–83–01–18826–9).

Full list of publications


  • 2020
  • 2019
  • 2018



Untitled designJune – come back to work during pandemic

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24-29 February – Dr hab. K.M. Marzec was invited to present the lecture about our results of red blood cell studies on the 8th International Conference on Perspectives in Vibrational Spectroscopy (ICOPVS-2020) in Bangalore, India


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6th of December – we had another meeting in order to discuss results carried out for LIDER project


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27th of September – we participated in Malopolska Science Night, during which Dr Dybaś, Dr Kaczmarska and M. Mrdyla tried to draw the visiting JCET children and young people into the magical red blood cells’ world.

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19th of September – we received the approval of the Bioethical Commission of Jagiellonian University for medical researches with cooperation Jagiellonian University Hospital.


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13-18 September – Dr hab. K.M. Marzec was invited to present the work carried out by our team on the 45th Congress of Polish Physicists, Krakow, Poland.

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2nd of September – we were invited to Jagiellonian University Hospital, were Dr Kaczmarska presented our current studies on Internal Seminar of Chair and Department of Hematology, Krakow, Poland.


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7-12 July – Dr hab. Marzec, Dr Dybas and Dr Kaczmarsak presented our current studies on 10th International Conference on Advanced Vibrational Spectroscopy ICAVS10, Auckland, New Zealand.

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17-18 June – we participated in Seminar Nano and Fast Vibrational Spectroscopy, Krakow, Poland organized by Raman Imaging Group from the Faculty of Chemistry. Our current studies were presented by Aneta Blat during oral presentation:

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6-8 June – we presented our current results on 27th Krakow Conference of Endothelium,
IX JCET Seminar in Male Ciche.

Untitled design30th of May – took place an official promotion of Dr Marzec habilitation:

The photo relation from the celebration can be found here.

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6th of May – we had a meeting in order to discuss results carried out for LIDER project:

Untitled design8th of January – Jakub Dybas had is PhD defense and he joined the society of doctors in RBCs Research Project Group.


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14th of December – we presented our results to SPARK-TECH and we have signed the agreement for future cooperation!


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30 of November – we have registered our trade mark! Hopefully we will be able to use it soon.



Untitled design26 of November – we had a meeting in order to discuss results carried out for LIDER project:

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20th of July – we had a meeting in order to discuss results carried out for OPUS project.



Untitled design1st of March – LIDER project started – let’s get to work!



Untitled design2nd of October – OPUS project started – let’s get to work!