TEAM

2

 

HEAD:
Katarzyna M. Marzec, PhD
Assistant Professor/Adjunct
katarzyna.marzec@jcet.eu
phone: (+48 12) 6635476

 

                                                                STAFF:

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Katarzyna Bulat, PhD
Postdoc/Assistant
katarzyna.bulat@jcet.eu
phone: (+48 12) 297 5472
room: B113

 

3

 

Magdalena Kaczmarska, PhD
Postdoc/Assistant
magadalena.kaczmarska@jcet.eu
phone: (+48 12) 297 5472
room: B113

 

6

 

Ewa Szczęsny-Małysiak, PhD
Postdoc/Assistant
ewa.szczesny@jcet.eu
phone: (+48 12) 297 5472
room: B113

 

4

 

Jakub Dybas, PhD
Postdoc/Assistant
jakub.dybas@jcet.eu
phone: (+48 12) 297 5472
room: B113

 

7

Aneta Blat, MSc, PhD student
aneta.blat@doctoral.uj.edu.pl
phone: +48 12 686 2391
Faculty of Chemistry, UJ
room: C3-23
PhD at Faculty of Chemistry, UJ
supervisor: dr hab. Kamilla Malek

 

1

 

Mateusz Mardyla, MSc
Technician
mateusz.mardyła@jcet.eu
phone: (+48 12) 297 5472
room: B113

 

We are a part of Raman Research Group at JCET and we cooperate closely with Raman Imaging Group at the Faculty of Chemistry UJ headed by Prof. dr hab. Małgorzata Baranska.

We cooperate closely with whole JCET team headed by Prof. dr hab. Stefan Chlopicki.
Until now, we have been cooperating mainly with dr Marek Grosicki, dr Kamil Kuś and Tasnim Mohaissen.

RESEARCH  PROJECTS

  1.  Diagnostics of the biochemical alterations in packed human red blood cells (PRBCs) with the use of vibrational spectroscopy
    Funding Agency: National Centre for Research and Development “Lider VIII”
  2. In search of biochemical, mechanical and functional fingerprints of oxidative stress in RBCs
    Funding Agency: National Science Centre, Poland, Program: OPUS

RESEARCH  PROFILE

  • Application of the spectroscopy supported by complementary techniques to human and murine RBCs studies
  • RBCs stress response on the biochemical, mechanical and functional level
  • Functional and dysfunctional hemoglobin adducts structure and analysis
  • RBCs membrane and cytoskeleton alterations
  • RBCs function and alterations in lifestyle diseases and in cancer metastasis
  • RBCs aging

CURRENT  RESEARCH  ACTIVITIES 

  • Studies of the biochemical, mechanical and functional fingerprints of oxidative stress in RBCs in different mice models of human lifestyle diseases
  • Development of the SPEC-DIAG-RED methodology for the application in human, packed RBCs diagnostic before transfusion

MAJOR  METHODS

  • Vibrational Spectroscopy:
    –  Raman and Resonance Raman spectroscopy (RS, RRS)
    –  Fourier-transform infrared spectroscopy (FT-IR)
  • Atomic force microscopy (AFM)
  • Scanning near-field optical microscope (SNOM)
  • UV–Vis absorption spectroscopy
  • Ektacytometry
  • Flow cytometry
  • Other classical techniques of blood and RBCs analysis (Blood gas analysis, Complete Blood counter, Morphology, Measurements of biochemical parameters etc.)

SELECTED  PUBLICATIONS

  1. D. Perez–Guaita, K.M. Marzec, A. Hudson, C. Evans, T. Chernenko, C. Matthäus, M. Miljkovic, M. Diem, P. Heraud, J. Richards, D. Andrew, D. Anderson, C. Doerig, J. Garcia–Bustos, D. McNaughton, B.R. Wood*, Parasites under the spotlight: Applications of vibrational spectroscopy to malaria research, Chem. Rev. 2018, 118, 5330–5358.
  2. J. Dybas, P. Berkowicz, B. Proniewski, K. Dziedzic–Kocurek, J. Stanek, M. Barańska, S. Chłopicki*, K.M. Marzec*, Spectroscopy–based characterization of Hb–NO adducts in human red blood cells exposed to NO–donor and endothelium–derived NO, Analyst 2018, 143, 4335–4346.
  3. J. Dybas, M. Grosicki, M. Barańska*, K.M. Marzec*, Raman imaging of haem metabolism in situ in macrophages and Kupffer cells, Analyst 2018, 143, 3489–3498.
  4. D. Perez–Guaita, M. de Veij, K.M. Marzec, A.R.D. Almohammedi, D. McNaughton, A.J. Hudson*, B.R. Wood*, Resonance Raman and UV–Visible Microscopy Reveals that Conditioning Red Blood Cells with Repeated Doses of Sodium Dithionite Increases Haemoglobin Oxygen Uptake, Chemistry Select 2017, 2 (11), 3342–3346.
  5. M. Kaczmarska, D. Zydek, J. Wiklacz-Potoczny, M. Fornal, T. Gordzicki, E. Kochowska, K. Kozak, L. Gocal, W. Pohorecki, K. Matlak, J. Korecki, K. Burda, Influence of very small doses of alpha radiation on the stability of erythrocytes, Microscopy.Res.Tech. 2017, 80 (1), 131-143
  6. K.M. Marzec*, J. Dybas, S. Chłopicki, M. Barańska, Resonance Raman in vitro detection and differentiation of the nitrite–induced hemoglobin adducts in functional human red blood cells, J. Phys. Chem. B 2016, 120, 12249–12260.
  7. S. Talu, S. Stach, M. Kaczmarska, M. Fornal, T. Grodzicki, W. Pohorecki, K. Burda*, Multifractal characterization of morphology of human red blood cells membrane skeleton, J.Microsc. 2016, 262(1), 59-72
  8. M. Kaczmarska, I. Habina, A. Orzechowska, K. Niemiec-Murzyn, M. Fornal, W. Pohorecki, K. Matlak, J. Korecki, T. Grodzicki, K. Burda*, Influence of neutron radiation on the stability of the erythrocyte membrane and oxyhemoglobin formation – Petkau effect studies, Acta Phys.Pol.B 2016, 47 (2), 425-440
  9. K.M. Marzec*, A. Rygula, B.R. Wood, S. Chłopicki, M. Barańska, High–resolution Raman imaging reveals spatial location of heme oxidation sites in single RBCs of dried smears, J. Raman Spectrosc. 2015, 46, 76–83.
  10. K.M. Marzec, D. Perez–Guaita, M. de Veij, D. McNaughton, M. Barańska, M.W.A. Dixon, L. Tilley, B.R. Wood*, Red blood cells polarize green laser light revealing hemoglobin’s enhanced non–fundamental Raman modes, Chem.Phys.Chem. 2014, 15, 3963–3968.
  11. M. Kaczmarska, M. Fornal, F.H. Messerli, J. Korecki, T. Grodzicki, K. Burda*, Erythrocyte membrane properties in patients with essential hypertension, Cell Biochem.Biophys. 2013, 67 (3), 1089-102
  12. K. Niemiec, M. Kaczmarska, M. Buczkowski, M. Fornal, W. Pohorecki, K. Matlak, J. Korecki, T. Gordzicki, K. Burda*, Mössbauer studies of hemoglobin in erythrocytes exposed to neutron radiation, Hyperfine Interact. 2012. 206 (1-3), 95-100
  13. M. Kaczmarska, Z. Kopyscinska, M. Fornal, T. Gordzicki, K. Matlak, J. Korecki, K. Burda*, Effects of low doses of gamma rays on the stability of normal and diabetic erythrocytes, Acta Biochim.Pol. 2011, 58 (4), 489-96
  14. K.M. Marzec*, J. Dybaś, Resonance Raman scattering spectroscopy in Vibrational spectroscopy. From theory to practice. red. K. Małek, Wydawnictwo Naukowe PWN SA, 2016, Warszawa, str. 46–52 (ISBN: 978–83–01–18885–6).
    Wersja w jęz. polskim:
    K.M. Marzec*, J. Dybaś, Spektroskopia rezonansowego rozpraszania ramanowskiego w Spektroskopia oscylacyjna. Od teorii do praktyki. red. K. Małek, Wydawnictwo Naukowe PWN SA, 2016, Warszawa, str. 47–53 (ISBN: 978–83–01–18826–9).
  15.  J. Dybaś, A. Chmura–Skirlińska, K.M. Marzec*, Resonance Raman scattering spectroscopy inhemoglobin structure studies in Vibrational spectroscopy. From theory to practice. red. K. Małek, Wydawnictwo Naukowe PWN SA, 2016, Warszawa, str. 185–192 (ISBN: 978–83–01–18885–6).
    Wersja w jęz. polskim:
    J. Dybaś, A. Chmura–Skirlińska, K.M. Marzec*, Spektroskopia rezonansowego rozpraszania ramanowskiego w badaniu struktury hemoglobiny w Spektroskopia oscylacyjna. Od teorii do praktyki. red. K. Małek, Wydawnictwo Naukowe PWN SA 2016, str. 191–198 (ISBN: 978–83–01–18826–9).

NEWS

  2019

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8th of January – Kuba had is PhD defense and he joined the society of doctors in RBCs Research Project Group.

 

2018

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14th of December – we presented our results to SPARK-TECH and we have signed the agreement for future cooperation!

 

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30 of November – we have registered our trade mark! Hopefully we will be able to use it soon.

 

 

Untitled design26 of November – we had a meeting in order to discuss results carried out for LIDER project:

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Untitled design1st of March – LIDER project started – let’s get to work!

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2017

Untitled design2nd of October – OPUS project started – let’s get to work!